CD38 Rat anti-Mouse, Brilliant Violet 605, Clone: 90/CD38 (also known as Ab90), BD Optibuild™

Description

• The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes
• In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells
• CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages
• Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells
• The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation
• CD31, both human and mouse, is reported to be a ligand for human CD38
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.