CD38 Rat anti-Mouse, Brilliant Violet 650, Clone: 90/CD38 (also known as Ab90), BD Optibuild™

Description

• The 90 monoclonal antibody specifically binds to CD38, a 42 kDa transmembrane glycoprotein on immature and mature, resting and activated, B lymphocytes
• In contrast to humans, CD38 expression is down-regulated on mouse germinal center B cells and plasma cells
• CD38 is also expressed on a subpopulation of thymic and peripheral T cells, NK cells, and splenic macrophages
• Furthermore, CD38 has been detected on bone marrow-derived hematopoietic stem cells
• The CD38 molecule is reported to exhibit both cyclase and hydrolase activities and plays a role in lymphocyte activation
• CD31, both human and mouse, is reported to be a ligand for human CD38
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.