CD49f Rat anti-Human, Brilliant Violet 605, Clone: GoH3, BD Optibuild™

Description

• The GoH3 monoclonal antibody specifically binds to CD49f which is also known as i ntegrin α6 chain
• CD49f is a ∽150 kDa type I transmembrane glycoprotein that belongs to the integrin alpha chain family of extracellular matrix and cell adhesion receptors
• The i ntegrin α6 subunit associates with the i ntegrin β1 chain (CD29) to form VLA-6 and with the integrin β4 chain (CD104) to form the integrin α6β4 complex, also known as the laminin and kalinin receptor
• CD49f is ex pressed mainly on T cells, monocytes, platelets, epithelial cells, endothelial cells, perineural cells, and trophoblasts of placenta
• GoH3 recognizes an extracellular epitope of integrin α6 on human, mouse and bovine cells
• GoH3 has been reported to block the binding of integrin α6 to laminin P1 and E8 fragments
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.