CD184 Mouse anti-Human, Brilliant Violet 605, Clone: 12G5, BD Optibuild™

Description

• The 12G5 monoclonal antibody specifically binds to CD184, also known as CXCR4 and Fusin
• CD184/CXCR4 is a seven-transmembrane domain, G-protein-linked, glycoprotein chemokine receptor
• CD184 serves as a receptor for the C-X-C chemokine, SDF-1
• It is expressed on a wide variety of hematopietic cells, vascular endothelial cells and cells of the nervous system
• CD184 plays a variety of roles in hematopoiesis, vascularization and neural development
• CD184 also functions as a coreceptor for infection with T-cell tropic strains of HIV-1 and as a receptor for CD4-independent infection by some HIV isolates
• The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T-cell tropic isolates of HIV-1
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407-nm and Em Max of 602-nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20-nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605-nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser.