CD184 Mouse anti-Human, Brilliant Violet 711, Clone: 12G5, BD Optibuild™

Description

• The 12G5 monoclonal antibody specifically binds to CD184, also known as CXCR4 and Fusin
• CD184/CXCR4 is a seven-transmembrane domain, G-protein-linked, glycoprotein chemokine receptor
• CD184 serves as a receptor for the C-X-C chemokine, SDF-1
• It is expressed on a wide variety of hematopietic cells, vascular endothelial cells and cells of the nervous system
• CD184 plays a variety of roles in hematopoiesis, vascularization and neural development
• CD184 also functions as a coreceptor for infection with T-cell tropic strains of HIV-1 and as a receptor for CD4-independent infection by some HIV isolates
• The 12G5 antibody has been reported to block CD4-independent infection by HIV-2 and CD4-dependent infection by some T-cell tropic isolates of HIV-1
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.