CD119 Rat anti-Mouse, Brilliant Violet 650, Clone: GR20, BD Optibuild™

Description

• The GR20 monoclonal antibody specifically binds to CD119, the α chain of the interferon γ(IFN- γ) receptor
• This receptor is expressed by mouse leukocytes, endothelial, and epithelial cells, but not by mature erythrocytes
• GR20 mAb recognizes an epitope (susceptible to certain fixatives) in the ligand-binding site of the receptor
• It can block the binding of mouse IFN- γ; therefore, it can inhibit IFN- γ.mediated effects including the activation of mouse macrophages for tumor cell killing
• IFN- γhas been reported to induce the expression of Ly-6A/E antigen on cell lines and normal B lymphocytes, and the GR20 antibody is capable of inhibiting the IFN- γ The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 650-nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.