CD71 Rat anti-Mouse, Brilliant Violet 711, Clone: C2 (also known as C2F2), BD Optibuild™

Description

• The C2 monoclonal antibody specifically binds to CD71, the transferrin receptor
• CD71 is a disulfide-linked homodimer of 95-kDa subunits
• CD71 mediates one of the cellular mechanisms for iron uptake, and its expression is regulated according to the cell’s iron requirements
• It is expressed at high levels on developing erythroid cells, and it is upregulated after mitogenic activation of B or T lymphocytes
• The C2 monoclonal antibody selectivity inhibits some types of T- and B-cell activation by down-regulation of transferrin receptor expression, but it does not block binding of transferrin
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 711-nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20-nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination.