CD11b Rat anti-Mouse, Brilliant Violet 786, Clone: M1/70, BD Optibuild™

Description

• The M1/70 monoclonal antibody specifically binds to CD11b, also known as Integrin alpha M (Itgam or αM)
• CD11b is a 170-kDa type 1 transmembrane glycoprotein and belongs to the Integrin alpha chain family
• CD11b serves as the alpha chain of the heterodimeric Mac-1 integrin (CD11b/CD18, αMβ2), also known as complement receptor 3 (CR3)
• Mac-1 mediates adhesion to ICAM-1 (CD54), ICAM-2 (CD102), fibrinogen and binding to C3bi
• Mac-1 is expressed at varying levels on granulocytes, macrophages, myeloid-derived dendritic cells, natural killer cells, microglia, and B-1 B lymphocytes
• Mac-1 expression is rapidly upregulated on neutrophils after activation, in the same time period that CD62L (L-selectin) is shed from the cell surface
• The M1/70 antibody reportedly blocks cell adherence and C3bi binding but does not block cell-mediated lysis
• Cross-reaction of the M1/70 antibody with CD11b expressed on human monocytes, polymorphonuclear leukocytes, and NK cells has been reported
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405-nm and an acceptor dye with an Em Max at 786-nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60-nm filter).