CD223 Rat anti-Mouse, BB700, Clone: C9B7W, BD Optibuild™

Description

• C9B7W mAb specifically binds to an epitope in the D2 domain of CD223 (LAG3), the 70kDa protein encoded by Lymphocyte-activation gene 3 (Lag3 )
• A fusion protein consisting of the entire extracellular region of mouse LAG3 with mouse IgG1 was used as immunogen
• CD223 is a type-I membrane protein with four extracellular Ig-like domains
• It is structurally homologous to CD4
• Like CD4, it binds MHC class II molecules
• Unlike CD4, it is not expressed on resting human and mouse T lymphocytes
• In mouse, as previously described in human, CD223 expression is upregulated on T lymphocytes (both CD4+ and CD8+) activated through the T-cell receptor (TCR) and on IL-2-activated NK (LAK) cells, and it is not detected on B cells, dendritic cells, or Phorbol 12-myristate 13-acetate (PMA)-stimulated splenocytes
• Studies on human peripheral T lymphocytes suggest that CD223 associates with the TCR to downregulate TCR signaling
• In contrast, in vivo and in vitro evaluations of vaccination protocols in mice suggest that CD223 promotes immune responses by activating antigen-presenting cells
• Furthermore, NK cells of Lag3-/-mice display defects in their capacity to kill certain tumor cells
• Mouse CD223 also has been demonstrated to contribute to the suppressor function of T regulatory cells and the C9B7W antibody has been shown to inhibit this function in vitro and in vivo
• Therefore, CD223 appears to play complex roles in the regulation of immune responses
• The antibody was conjugated to BD Horizon™ BB700, which is part of the BD Horizon Brilliant™ Blue family of dyes
• It is a polymer-based tandem dye developed exclusively by BD Biosciences
• With an excitation max of 485 nm and an emission max of 693 nm, BD Horizon BB700 can be excited by the 488 nm laser and detected in a standard PerCP-Cy™5.5 set (eg, 695/40nm filter)
• This dye provides a much brighter alternative to PerCP-Cy5.5 with less cross laser excitation off the 405 nm and 355 nm lasers.BD Horizon Brilliant™ Blue 700 (BB700) is a dye that was exclusively developed by BD Biosciences as brighter alternative to PerCP-Cy5.5
• This dye also has less cross laser excitation off the 405 nm laser, resulting in less spillover into the violet channels compared to PerCP-Cy5.5.  Due to similar excitation and emission properties, BD Horizon BB700 and PerCP-Cy5.5 cannot be used simultaneously.