CD97 Mouse anti-Human, Brilliant Violet 421, Clone: VIM3b, BD Optibuild™

Description

• The VIM3b monoclonal antibody recognizes CD97
• CD97 is a seven-span transmembrane protein that belongs to the G-protein coupled receptor superfamily
• CD97 is encoded by ADGRE5 (adhesion G protein-coupled receptor E5)
• CD97 is highly expressed on monocytes and granulocytes
• It is also expressed on dendritic cells, T cells, B cells, NK cells, and smooth muscle cells
• CD97 expression is upregulated on activated T cells
• CD97 is also expressed on several cell lines including HL-60, THP-1, and K562
• CD97 reportedly plays roles in leucocyte signaling, adhesion, and migration
• The VIM3b antibody works in Western blotting and immunoprecipitation
• The antibody recognizes proteins of 74, 80 and 89 kDa
• CD55 and chondroitin sulfate are ligands for CD97
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.