CD11b Mouse anti-Human, Brilliant Violet 605, Clone: D12, BD Optibuild™

Description

• The D12 monoclonal antibody specifically binds to CD11b which is also known as Integrin alpha M (IntegrinαM), Mac-1 subunit alpha (Mac-1a) or complement receptor 3 alpha chain (CR3a)
• CD11b is encoded by ITGAM /(integrin subunit alpha M) and belongs to the integrin alpha subunit gene family
• CD11b is expressed as a ñ165-kDa type I transmembrane glycoprotein that associates with the ñ95-kDa integrin β2(CD18) to form the heterodimeric CD11b/CD18 (αM/β2) complex which also known as Mac-1 or CR3
• CD11b is expressed on monocytes, dendritic cells, granulocytes, as well as on some T cells, B cells, and NK cells
• CD11b functions in cell-cell and cell-substrate interactions and is a receptor for multiple ligands including inactivated C3b (iC3b), ICAM-1 (CD54), ICAM-2 (CD102), ICAM-3 (CD50) or fibrinogen
• Mac-1 regulates leucocyte adhesion and migration, as well as phagocytosis of opsonized particles
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.