CD4 v4 Mouse anti-Human, Brilliant Violet 605, Clone: L120, BD Optibuild™

Description

• The L120 monoclonal antibody specifically recognizes CD4
• CD4 is an ñ59 kDa type I transmembrane glycoprotein that belongs to the Ig gene superfamily
• CD4 is expressed on helper-inducer subset of T lymphocytes and monocytes
• The CD4 antigen is involved in the Major Histocompatibility Complex (MHC) class II-restricted recognition of foreign antigens and has been identified as a receptor for HIV
• The L120 antibody recognizes amino acids 306-370 of the antigen and does not block the binding of the gp120 of HIV, but inhibits the proliferation of CD4 T lymphocytes to MHC class II antigens
• The CD4 antigen is present on most thymocytes, approximately 40% of peripheral blood lymphocytes, and at lower density on monocytes
• Peripheral blood CD4+ T lymphocytes are lost over time during HIV infection, and this loss is correlated with disease progression
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.