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BDB742830

CD277 Mouse anti-Human, Brilliant Violet 711, Clone: 232-5, BD Optibuild™

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB742830-Each-of-1 In Stock ₹ 47,400.24

BDB742830 - Each of 1

₹ 47,400.24

In Stock

Quantity

1

Base Price: ₹ 47,400.24

GST (18%): ₹ 8,532.043

Total Price: ₹ 55,932.283

Antigen

CD277

Clone

232-5

Conjugate

Brilliant Violet 711

Gene Alias

CD277, Butyrophilin 3, BTN3, BTN3A1, BTN3A2, BTN3A3, BTN3.1, BTN3.2, BTN3.3

Immunogen

Human BTN3A3

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Human

Isotype

IgG2a κ

Description

  • The 232-5 monoclonal antibody specifically recognizes Butyrophilin 3 (BTN3), which is also known as CD277
  • BTN3 is comprised of three structurally related members, BTN3A1, BTN3A2, and BTN3A3
  • BTN3 molecules are transmembrane type 1 glycoproteins that belong to the Ig superfamily
  • The 232-5 antibody recognizes the variable region of the extracellular domains of BTN3 molecules
  • BTN3 molecules are differentially expressed by T, NKT, B, and NK cells, monocytes, macrophages, dendritic cells, as well as some stem cells and tumor cells
  • BTN3 expression is decreased upon lymphocyte activation
  • The extracellular domain of CD277 is structurally related to the B7 family of proteins that regulate T cell activation
  • A soluble form of the 232-5 antibody can serve as an agonist in BTN3-mediated regulation of anti-CD3±anti-CD28 antibody activated CD4+ or CD8+ T cells
  • The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
  • BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • However, the spillover can be corrected through compensation as with any other dye combination
  • BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
  • This dye offers a very bright choice for the violet laser
  • Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
  • BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.

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Img

BD Biosciences

BDB742830

--

Antigen:
CD277
Clone:
232-5
Conjugate:
Brilliant Violet 711
Gene Alias:
CD277, Butyrophilin 3, BTN3, BTN3A1, BTN3A2, BTN3A3, BTN3.1, BTN3.2, BTN3.3
Immunogen:
Human BTN3A3
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG2a κ

Img

BD Biosciences

BDB742831

--

Antigen:
CD277
Clone:
232-5
Conjugate:
Brilliant Violet 786
Gene Alias:
CD277, Butyrophilin 3, BTN3, BTN3A1, BTN3A2, BTN3A3, BTN3.1, BTN3.2, BTN3.3
Immunogen:
Human BTN3A3
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG2a κ

Img

BD Biosciences

BDB742832

--

Antigen:
CD277
Clone:
232-5
Conjugate:
Brilliant Ultraviolet 395
Gene Alias:
CD277, Butyrophilin 3, BTN3, BTN3A1, BTN3A2, BTN3A3, BTN3.1, BTN3.2, BTN3.3
Immunogen:
Human BTN3A3
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG2a κ

Img

BD Biosciences

BDB742834

--

Antigen:
Ig, κ light chain
Clone:
187.1
Conjugate:
Brilliant Violet 510
Gene Alias:
Ig kappa chain C region, IGKC, Igk-C, Ig, κ
Immunogen:
Mouse IgG2b κ secreted by MPC-11 plasmacytoma
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Rat
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Mouse
Isotype:
IgG1 κ