CD8b Mouse Anti-Rat, Brilliant Violet 711, Clone: 341, BD Optibuild™

Description

• The 341 monoclonal antibody specifically recognizes theβchain of the CD8 antigen on most thymocytes and a subpopulation of mature T lymphocytes (ie, MHC class I-restricted T cells, including most T suppressor/cytotoxic cells)
• The CD8αandβchains (CD8a and CD8b, respectively) form a heterodimer on the surface of most thymocytes and thymus-dependent T suppressor/cytotoxic cells, whereas intestinal intraepithelial lymphocytes, many CD8+ T cells of athymic rats, many activated CD4+ T cells, and most NK cells express CD8a without CD8b
• It has been suggested that the expression of the CD8a/CD8b heterodimer is restricted to thymus-derived T lymphocytes
• CD8 is an antigen co-receptor on the T cell surface which interacts with MHC class I molecules on antigen-presenting cells
• It participates in T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase lck
• Macrophages have also been reported to express CD8αandβchains, which are involved in signal transduction
• The 341 mAb blocks proliferative and cytotoxic in vitro responses of CD8+ effectors to allogeneic cells
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.