CD8 Mouse anti-Human, Brilliant Violet 650, Clone: G42-8, BD Optibuild™

Description

• The G42-8 monoclonal antibody specifically recognizes the alpha subunit (32 kDa) of CD8ααhomodimer or CD8αβheterodimer
• The CD8 molecule binds to HLA class I molecules during interaction of CD8+ T cells with antigen-presenting cells or target cells
• CD8 is expressed on T cytotoxic/suppressor cell populations
• G42-8 stains approximately 13-48% of peripheral blood lymphocytes and 80% of thymocytes, as well as a subset of NK cells
• This antibody is useful in detecting CD8+ T cells when using DimerX I reagents
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.