CD151 Mouse anti-Human, Brilliant Violet 786, Clone: 14A2.H1, BD Optibuild™

Description

• Reacts with platelet-endothelial cell tetraspan antigen-3 (PETA-3), a 27 kD membrane glycoprotein, expressed on platelets, megakaryocytes, lymphocytes (weak), monocytes, endothelial cells and epithelial cells
• PETA-3 (CD151) associates withβ1 integrin in certain tissues
• This has also been shown with other tetraspan superfamily members, like CD9, CD63 andα5β1
• Reports indicate that this association or colocalization of CD151 withβ1 integrin in tissues suggests a functional role of this molecule, however, this role has not been elucidated yet
• It has also been reported that antibody 14A2.H1 is capable of platelet activation in vitro.Studies showed that different clones of CD151 monoclonal antibodies display strikingly different patterns of binding to human haemopoietic cells and tissue sections, and that this is due at least in part to the presence of the protein in complexes with different integrins
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter)
• BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm
• BV786 offers a bright choice for the 6th detector off the violet laser.