CD119 Mouse anti-Human, BUV395, Clone: GIR-208, BD Optibuild™

Description

• The GIR-208 antibody recognizes the extracellular region of CD119 which is also known as the alpha chain subunit (80-95 kDa glycoprotein) of the human interferon-γreceptor (IFN-γRα)
• The functionally active-form of the human IFN-γreceptor consists of two (or more) subunits, with IFN-γRαresponsible for IFN-γbinding and both the IFN-γRαandβchains required for the transduction of biologic responses
• The IFN-γreceptorαchain (CD119) is expressed on the surface of most human cells (except mature erythrocytes) including monocytes, macrophages, T cells, B cells, NK cells, neutrophils, fibroblasts, epithelial cells, and endothelium
• Binding of 125I-labeled GIR-208 antibody to IFN-γRα+ cells is reported to be specifically inhibited in the presence of excess IFN-γ
• GIR-208 does not cross react with IFN-γas tested by ELISA
• The ability of this antibody to bind to IFN-γreceptors of species other than human has not been determined
• The immunogen used to generate this hybridoma was human IFN-γ The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.