CD140b Mouse anti-Human, Brilliant Violet 510, Clone: 28D4, BD Optibuild™

Description

• Reacts with platelet derived growth factor (PDGF) receptorβ, a 170-190 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes
• PDGF receptors,αandβ, are single glycoproteins with intracellular tyrosine kinase domains
• They are structurally similar to M-CSF receptor and CD117 (c-kit)
• Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells
• PDGF plays a role in wound healing and acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils
• Functional PDGF is secreted as a disulfide-linked dimer of A and B chains (PDGF-AA, PDGF-BB or PDGF-AB)
• Binding of divalent PDGF induces receptor dimerization with three possible forms:αα,αβ,ββ
• The PDGF receptorα-subunit binds both PDGF A and B chains, whereas the receptorβ-subunit binds only PDGF B chain
• Both receptor subunits can stimulate mitogenic responses, only theβsubunit can induce chemotaxis
• 28D4 is specific for PDGFRβand does not cross-react with PDGFRα
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.