CD140a Mouse anti-Human, Brilliant Violet 605, Clone: αR1 (also known as Alpha-R1), BD Optibuild™

Description

• The αR1 monoclonal antibody specifically binds to the human platelet derived growth factor (PDGF) receptor α (PDGFRα), also known as CD140a
• CD140a is a 170 kDa single transmembrane glycoprotein expressed on fibroblasts, smooth muscle cells, glial cells and chondrocytes
• PDGF receptors α and βare single glycoproteins with intracellular tyrosine kinase domains
• They are structurally similar to the M-CSF receptor and CD117 (c-kit)
• Their ligand, PDGF, is a mitogen for connective tissue cells and glial cells
• PDGF plays a role in wound healing and it also acts as a chemoattractant for fibroblasts, smooth muscle cells, glial cells, monocytes and neutrophils
• Functional PDGF is secreted in disulfide linked, homodimeric or heterodimeric forms comprised of A or B chains (PDGFAA, PDGF-BB or PDGF-AB)
• Binding of divalent PDGF induces receptor dimerization with three possible forms: αα, αβ, ββ
• The PDGFRαsubunit binds both PDGF A and B chains, whereas the PDGFRβ subunit binds only PDGF B chains
• Although both receptor subunits can stimulate mitogenic responses, only the β subunit can induce chemotaxis.TheαR1antibody is specific for PDGFRαand does not crossreact with PDGFRβ
• It immunoprecipitates human, monkey, rabbit, pig, dog and cat PDGFRα
• It does not recognize hamster, rat or mouse PDGFRα
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.