CD116 Mouse anti-Human, Brilliant Violet 711, Clone: hGMCSFR-M1, BD Optibuild™

Description

• The hGMCSFR-M1 antibody reacts with the subunit (GM-CSFR) of the human Granulocyte-Macrophage Colony-Stimulating Factor Receptor complex
• This 75-85 kD subunit is also known as CD116
• The hGMCSFR-M1 antibody was first clustered at the Fifth International Workshop on Human Leucocyte Differentiation Antigens
• The GM-CSFR subunit associates with the 120-140 kDβc subunit (common subunit, CD131), that is shared with the receptors for interleukins IL-3 and IL-5
• Both of the chains of the GM-CSFR complex are involved in ligand binding and intracellular signaling
• Theαchain appears to transmit most of the biological signals
• CD116 is expressed by a variety of myeloid cell lines, hematopoietic and non-hematopoetic tumor cells, and normal cell types including monocytes, macrophages, neutrophils, eosinophils, myeloid dendritic cells, endothelial cells, fibroblasts, and placental trophoblasts
• Lymphocytes are negative for GM-CSFR expression
• Reports suggest that GM-CSFR plays a role in myeloid lineage growth and differentiation
• The immunogen used to generate the hGMCSFR-M1 hybridoma was recombinant human GM-CSFR
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.