c-Met Mouse anti-Human, Brilliant Violet 510, Clone: 3D6, BD Optibuild™

Description

• The 3D6 monoclonal antibody specifically binds to c-Met (MET), which is also known as Hepatocyte growth factor receptor (HGFR) or Scatter factor receptor (SF receptor)
• c-Met is a 190 kDa single-pass type I transmembrane glycoprotein that is posttranslationally cleaved into a disulfide-linked extracellularα-chain and a transmembraneβ-chain
• c-Met functions as a receptor tyrosine kinase (RTK) that is normally involved in the development, regeneration, and survival of cells and tissues
• c-Met is expressed on a variety of cell types including stem cells and progenitor cells, hepatocytes, keratinocytes, epithelial cells, endothelial cells, and neurons
• c-Met is autophosphorylated when bound by its ligand, Hepatocyte Growth Factor (HGF)
• This leads to further activation of downstream signaling pathways that induce multiple responses including cellular migration, proliferation, survival, and angiogenesis
• Abnormal c-Met expression or activity has been associated with tumorigenesis
• The 3D6 antibody can reportedly function as an agonist by binding to and activating human but not mouse c-Met
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.