CD114 Mouse anti-Human, Brilliant Violet 711, Clone: LMM741, BD Optibuild™

Description

• The LMM741 monoclonal antibody specifically recognizes CD114 which is also known as the Granulocyte-Colony Stimulating Factor Receptor (G-CSFR)
• CD114 is a ñ150 kDa type I transmembrane glycoprotein that is encoded by CSF3R (colony stimulating factor 3 receptor) and belongs to the class 1 cytokine receptor family
• CD114 is expressed on granulocytes, monocytes, dendritic cells, endothelial cells, platelets, placenta and myeloid leukemias and a variety of tumor cell lines
• CD114 serves as the receptor for granulocyte colony stimulating factor (G-CSF) which plays a role in myeloid cell proliferation and differentiation
• The immunogen used to generate this hybridoma was cells transfected with an expression vector containing a full-length cDNA encoding the human G-CSFR
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.