CD114 Mouse anti-Human, BUV395, Clone: LMM741, BD Optibuild™

Description

• The LMM741 monoclonal antibody specifically recognizes CD114 which is also known as the Granulocyte-Colony Stimulating Factor Receptor (G-CSFR)
• CD114 is a ñ150 kDa type I transmembrane glycoprotein that is encoded by CSF3R (colony stimulating factor 3 receptor) and belongs to the class 1 cytokine receptor family
• CD114 is expressed on granulocytes, monocytes, dendritic cells, endothelial cells, platelets, placenta and myeloid leukemias and a variety of tumor cell lines
• CD114 serves as the receptor for granulocyte colony stimulating factor (G-CSF) which plays a role in myeloid cell proliferation and differentiation
• The immunogen used to generate this hybridoma was cells transfected with an expression vector containing a full-length cDNA encoding the human G-CSFR
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.