CD162 Mouse anti-Human, Brilliant Violet 421, Clone: KPL-1, BD Optibuild™

Description

• The KPL-1 monoclonal antibody specifically binds to p-selectin glycoprotein ligand-1 (PSGL-1), a 120-220 kDa, mucin-like type I transmembrane glycoprotein
• CD162 is expressed on neutrophils, monocytes, and most lymphocytes
• CD162 binds to CD62P (P-Selectin), CD62E (E-Selectin) and CD62L (L-Selectin)
• This molecule mediates rolling of leukocytes on activated endothelium and the interaction between leukocytes and activated platelets or other leukocytes found at inflammatory sites
• It has also been reported that this clone completely inhibits the interaction between P-Selectin and either CD4-T cells or neutrophils
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.