CD114 Mouse anti-Human, Brilliant Violet 510, Clone: LMM741, BD Optibuild™

Description

• The LMM741 monoclonal antibody specifically recognizes CD114 which is also known as the Granulocyte-Colony Stimulating Factor Receptor (G-CSFR)
• CD114 is a ñ150 kDa type I transmembrane glycoprotein that is encoded by CSF3R (colony stimulating factor 3 receptor) and belongs to the class 1 cytokine receptor family
• CD114 is expressed on granulocytes, monocytes, dendritic cells, endothelial cells, platelets, placenta and myeloid leukemias and a variety of tumor cell lines
• CD114 serves as the receptor for granulocyte colony stimulating factor (G-CSF) which plays a role in myeloid cell proliferation and differentiation
• The immunogen used to generate this hybridoma was cells transfected with an expression vector containing a full-length cDNA encoding the human G-CSFR
• The antibody was conjugated to BD Horizon™ BV510 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 405nm and Em Max at 510nm, BD Horizon BV510 can be excited by the violet laser and detected in the BD Horizon V500 (525/50nm) filter set
• BD Horizon BV510 conjugates are useful for the detection of dim markers off the violet laser
• BV510 is a polymer-based dye that is brighter than BD Horizon V500
• Due to similar excitation and emission properties, BD Horizon BV510 and BD Horizon V500 cannot be used simultaneously.