B7-H4 Mouse anti-Human, Brilliant Violet 605, Clone: MIH43, BD Optibuild™

Description

• The MIH43 monoclonal antibody specifically binds to human B7-H4 (B7 family member, H4) that is encoded by the VTCN1 (V-set domain containing T cell activation inhibitor 1) gene
• B7-H4 is a type I membrane glycoprotein with a calculated molecular weight of 30.89 kDa
• It is also known as VCTN1, B7h.5, B7S1 (B7 superfamily member 1) or B7X
• B7-H4 is a newly discovered member of the B7 family of costimulatory proteins
• B7-H4 is not constitutively expressed on peripheral tissues
• Its expression can be induced on macrophages, dendritic cells, B cells and T cells
• By binding to its putative receptor, the function of B7-H4 was initially reported to be a negative regulator of T cell activation, proliferation and differentiation related to cytokine production and cytotoxic effector cell functions
• B7-H4 is reportedly overexpressed in a variety tumors
• B7-H4 expression by tumor macrophages appears to play a role in suppression antigen-specific T cell mediated immunity
• Recent studies indicate that B7-H4 can also be a positive regulator of T cell responses
• B7-H4 can be involved innate immunity as well, eg, by inhibiting neutrophil expansion
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.