CD116 Mouse anti-Human, BUV395, Clone: hGMCSFR-M1, BD Optibuild™

Description

• The hGMCSFR-M1 antibody reacts with the subunit (GM-CSFR) of the human Granulocyte-Macrophage Colony-Stimulating Factor Receptor complex
• This 75-85 kD subunit is also known as CD116
• The hGMCSFR-M1 antibody was first clustered at the Fifth International Workshop on Human Leucocyte Differentiation Antigens
• The GM-CSFR subunit associates with the 120-140 kDβc subunit (common subunit, CD131), that is shared with the receptors for interleukins IL-3 and IL-5
• Both of the chains of the GM-CSFR complex are involved in ligand binding and intracellular signaling
• Theαchain appears to transmit most of the biological signals
• CD116 is expressed by a variety of myeloid cell lines, hematopoietic and non-hematopoetic tumor cells, and normal cell types including monocytes, macrophages, neutrophils, eosinophils, myeloid dendritic cells, endothelial cells, fibroblasts, and placental trophoblasts
• Lymphocytes are negative for GM-CSFR expression
• Reports suggest that GM-CSFR plays a role in myeloid lineage growth and differentiation
• The immunogen used to generate the hGMCSFR-M1 hybridoma was recombinant human GM-CSFR
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.