CD193 Mouse anti-Human, Brilliant Violet 650, Clone: 5E8 (also known as 5E8-G9-B4), BD Optibuild™

Description

• The 5E8 monoclonal antibody specifically binds to human CCR3 which is also known as CD193
• CCR3 is a G protein-linked, 7 transmembrane, chemokine receptor expressed on a variety of hematopoietic cells
• Similar to CCR5 and CXCR4, CCR3 can be a co-receptor for HIV-1
• It is primarily expressed by eosinophils and basophils during atopic conditions, dermatitis, allergic rhinitis, conjunctivitis and bronchial asthma
• Chemokines including RANTES, Eotaxin, MCP-3, MIP1αhave been reported to act as ligands for CCR3 and stimulate CCR3+ cells
• Eotaxin stimulates Th2 cells expressing CCR3
• Other studies describe HIV-1 specific T cell cytotoxicity can be mediated by RANTES and Eotaxin through CCR3
• CCR3 expressed on dendritic cells may have a biological role on cell-cell interaction during antigen presentation
• CCR3 has been clustered as CD193 in the HLDA VIIIth workshop
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.