Vγ 3 TCR Hamster anti-Mouse, Brilliant Violet 421, Clone: 536, BD Optibuild™

Description

• The 536 monoclonal antibody specifically recognizes Vγ3† T-cell Receptor (TCR)-bearing T lymphocytes, which are the predominantγδTCR-bearing cells in the early fetal thymus and the adult epidermis of euthymic mice
• The first T cells to mature in the embryonic thymus express the Vγ3 and Vδ1 TCR chains, and their development is dependent upon IL-7
• There is evidence that the Vγ3 TCR-bearing fetal thymocytes are the precursors of the majority of dendritic epidermal T cells (DEC), which may be replenished in the adult by proliferation in situ /rather than by seeding from primary lymphoid organs
• Although the Vγ3 TCR is almost exclusively found in the DEC population, it has been shown that the homing of DEC to the epidermis does not require expression of the Vγ3 gene segment
• Vγ3 TCR-bearing dermal dendritic cells have also been described
• Vγ3 TCR has also been found on a subset of T lymphocytes in the lactating mammary gland and at the site of antigenic challenge in contact-sensitized mice
• Plate-bound 536 antibody activates Vγ3 TCR-bearing T cells, and Fab fragments of mAb 536 block in vitro /stimulation of DEC by keratinocytes
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.