Vγ 3 TCR Hamster anti-Mouse, Brilliant Violet 605, Clone: 536, BD Optibuild™

Description

• The 536 monoclonal antibody specifically recognizes Vγ3† T-cell Receptor (TCR)-bearing T lymphocytes, which are the predominantγδTCR-bearing cells in the early fetal thymus and the adult epidermis of euthymic mice
• The first T cells to mature in the embryonic thymus express the Vγ3 and Vδ1 TCR chains, and their development is dependent upon IL-7
• There is evidence that the Vγ3 TCR-bearing fetal thymocytes are the precursors of the majority of dendritic epidermal T cells (DEC), which may be replenished in the adult by proliferation in situ /rather than by seeding from primary lymphoid organs
• Although the Vγ3 TCR is almost exclusively found in the DEC population, it has been shown that the homing of DEC to the epidermis does not require expression of the Vγ3 gene segment
• Vγ3 TCR-bearing dermal dendritic cells have also been described
• Vγ3 TCR has also been found on a subset of T lymphocytes in the lactating mammary gland and at the site of antigenic challenge in contact-sensitized mice
• Plate-bound 536 antibody activates Vγ3 TCR-bearing T cells, and Fab fragments of mAb 536 block in vitro /stimulation of DEC by keratinocytes
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.