VISTA Rat anti-Mouse, Brilliant Violet 711, Clone: MIH64, BD Optibuild™

Description

• The MIH64 monoclonal antibody specifically binds to V-domain Ig suppressor of T cell activation (VISTA) which is also known as, Differentiation of ESCs 1 (Dies1)
• VISTA is a type I transmembrane glycoprotein that belongs to the Ig superfamily
• VISTA has homology to the B7 family ligand, PD-L1 (CD274), and is likewise known as PD-1H
• VISTA is expressed on naοve and activated T cells, natural killer (NK) cells, macrophages, dendritic cells, and neutrophils
• VISTA functions as an inhibitory ligand
• It can serve as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation
• It is also involved in regulating stem cell differentiation
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.