TCR Vβ8 Mouse anti-Human, Brilliant Violet 711, Clone: JR2, BD Optibuild™

Description

• The JR2 monoclonal antibody specifically recognizes the -chain variable region (V 8) of the T-cell antigen receptor expressed on 0.5% to 3% of peripheral blood T cells
• This antibody has been further characterized as having specificity to a major subset of the BV8 region as determined in the TCR Workshop of July 1995
• Staphylococcal enterotoxin E has been shown to specifically stimulate T cells bearing V 8 (BV8)
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.