TCR Vβ3 Hamster anti-Mouse, Brilliant Violet 711, Clone: KJ25, BD Optibuild™

Description

• The KJ25 antibody specifically reacts with the V β 3 T-cell Receptor (TCR) of strains having the a (e.g., C57BR, SJL), b (e.g., AKR, CBA/Ca, C57BL, DBA/1), and c (e.g., RIII) haplotypes of the Tcrb gene complex
• V β 3 TCR-bearing T lymphocytes are clonally eliminated either completely or partially in mice expressing superantigens encoded by the Mtv-1 ( Mls-4[a], Mls[c] ), Mtv-3 ( Mls[c] ), Mtv-6 ( Mls-3[a], Mls[c] ), Mtv-13 ( Mls-2[a], Mls[c] ), Mtv-27, Mtv-44, and/or Mtv-MAI endogenous proviruses (e.g., A, BALB/c, CBA/J, C3H/He, DBA/2, NZB, NZW)
• V β 3 TCR-bearing T cells are activated by the superantigenic Staphylococcal Enterotoxins A and B
• Activation or elimination of V β 3 TCR-expressing T cells by these determinants is partially dependent upon presentation by I-E
• This hamster mAb to a mouse leukocyte antigen does not cross-react with rat leukocytes
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.