Vβ 8 T-Cell Receptor Mouse anti-Mouse, Brilliant Violet 786, Clone: F23.1, BD Optibuild™

Description

• The F23.1 antibody specifically reacts with the V β 8.1, V β 8.2, and V β 8.3 T-cell receptors (TCR) of mice having the b haplotype ( e.g., A, AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C58, DBA/1, DBA/2) of the Tcrb gene complex
• The Tcrb-V8 subfamily gene loci are deleted in mice having the a ( e.g., C57BR, C57L, SJL, SWR) or c ( e.g., RIII) haplotype
• V β 8.1 TCR-bearing T lymphocytes are clonally eliminated in mice expressing superantigen coded by Mtv-7 (Mls-1a, Mlsa) provirus ( e.g., AKR, CBA/J, C58, DBA/2), and activation or elimination of V β 8.1 TCR-expressing T cells by this determinant is partially dependent upon presentation by I-E
• Mtv-43 and/or exogenous MMTV-SW superantigens also cause incomplete elimination of V β 8.1 TCR-bearing T cells
• In addition to expression on conventional T lymphocytes, V β 8.2 is the predominant β chain of the TCR on NK-T cells
• Staphylococcal enterotoxin B, in association with antigen-presenting cells expressing I-A and/or I-E, stimulates lymphocytes bearing V β 8 TCR and selectively eliminates those T cells in vivo
• Soluble and plate-bound F23.1 antibody activates V β 8 TCR-bearing T cells, soluble antibody blocks cytolysis mediated by V β 8 TCR-bearing cytotoxic T lymphocytes, and in vivo treatment of neonatal mice can arrest intrathymic maturation of V β 8 TCR-bearing T cells
• The antibody was conjugated to BD Horizon™ BV786 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 786nm
• BD Horizon BV786 can be excited by the violet laser and detected in a filter used to detect Cy™7-like dyes (eg, 780/60nm filter)
• BV786 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 786 nm
• BV786 offers a bright choice for the 6th detector off the violet laser.