TCR Vβ8 Mouse anti-Human, Brilliant Violet 421, Clone: JR2, BD Optibuild™

Description

• The JR2 monoclonal antibody specifically recognizes the -chain variable region (V 8) of the T-cell antigen receptor expressed on 0.5% to 3% of peripheral blood T cells
• This antibody has been further characterized as having specificity to a major subset of the BV8 region as determined in the TCR Workshop of July 1995
• Staphylococcal enterotoxin E has been shown to specifically stimulate T cells bearing V 8 (BV8)
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.