BDB743341

Marginal Zone B Cells Mouse Anti-Rat, Brilliant Violet 421, Clone: HIS57, BD Optibuild™

Manufacturer: BD Biosciences

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Pack Size SKU Availability Price
Each of 1 BDB743341-Each-of-1 In Stock ₹ 34,052.88

BDB743341 - Each of 1

₹ 34,052.88

In Stock

Quantity

1

Base Price: ₹ 34,052.88

GST (18%): ₹ 6,129.518

Total Price: ₹ 40,182.398

Antigen

Marginal Zone B Cells

Clone

HIS57

Conjugate

Brilliant Violet 421

Host Species

Mouse

Purification Method

Affinity Purified

Regulatory Status

RUO

Target Species

Mouse

Isotype

IgG1 κ

Classification

Monoclonal

Concentration

0.2mg/mL

Formulation

Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen

Rat Splenic B Cells

Quantity

50 μg

Primary or Secondary

Primary

Content And Storage

Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Description

  • The HIS57 monoclonal antibody reacts with an unknown antigen that is highly expressed by most marginal zone B (MZ-B) cells in the spleen
  • In contrast, this antigen is weakly expressed, or not expressed at all, by other B-cell subpopulations
  • Rat MZ-B cells express low levels of CD45R (mAb HIS24) and sIgD and high levels of sIgM
  • The HIS57 mAb does not stain granulocytes and thymocytes
  • Immunohistochemical staining of normal spleen sections with HIS57 mAb produced a positive signal in the marginal zone and, to a lesser extent, in B-cell follicles
  • This marker can be used in combination with CD45R, sIgD, and sIgM t0 identify MZ-B cells in the rat
  • The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
  • With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
  • BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
  • BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
  • Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
  • Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.

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BD Biosciences

BDB743341

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Antigen:
Marginal Zone B Cells

Clone:
HIS57

Conjugate:
Brilliant Violet 421

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Rat Splenic B Cells

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

BD Biosciences

BDB743343

--


Antigen:
Marginal Zone B Cells

Clone:
HIS57

Conjugate:
Brilliant Violet 605

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Rat Splenic B Cells

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

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BD Biosciences

BDB743344

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Antigen:
Marginal Zone B Cells

Clone:
HIS57

Conjugate:
Brilliant Violet 650

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Rat Splenic B Cells

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.

Img

BD Biosciences

BDB743345

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Antigen:
Marginal Zone B Cells

Clone:
HIS57

Conjugate:
Brilliant Violet 711

Host Species:
Mouse

Purification Method:
Affinity Purified

Regulatory Status:
RUO

Target Species:
Mouse

Isotype:
IgG1 κ

Classification:
Monoclonal

Concentration:
0.2mg/mL

Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.

Immunogen:
Rat Splenic B Cells

Quantity:
50 μg

Primary or Secondary:
Primary

Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.