CD271 Mouse anti-Human, Brilliant Violet 711, Clone: C40-1457, BD Optibuild™

Description

• The C40-1457 monoclonal antibody specifically recognizes CD271 that is also known as the nerve growth factor receptor (NGFR)
• CD271 is 75 kDa type I transmembrane glycoprotein likewise known as TNFRSF16 that belongs to the tumor necrosis factor receptor (TNFR) superfamily
• CD271 has been found localized to neuronal axons, Schwann cells, and perineural cells of peripheral nerves
• It is also expressed by some epithelial, mesenchymal and lymphoid tissues
• NGFR is the receptor for nerve growth factor (NGF), a polypeptide that is essential for normal development of the nervous system
• NGF promotes survival and differentiation of sympathetic and sensory neurons during embryological development of the peripheral nervous system
• NGF binds to two distinctive surface receptors, the p/140[prototrk] and p75[NGFR]
• High affinity binding of NGF requires that both receptor molecules be expressed
• NGFR is expressed on human and rat lymphocytes
• A subset of lymphoid cells in the spleen, lymph nodes, and follicular dendritic cells in germinal centers of reactive lymph nodes were found to express CD271
• It has been reported that NGFR interaction with its ligand, NGF, may play a role in immunoregulation
• NGF may also function as a B-cell growth factor The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.