CD329 (Siglec-9) Mouse anti-Human, Brilliant Violet 650, Clone: E10-286, BD Optibuild™

Description

• The E10-286 monoclonal antibody specifically binds to CD329
• CD329 is also known as Sialic acid-binding Ig-like lectin 9 (Siglec-9), formerly known as Siglec-Y, which is expressed on peripheral blood monocytes, granulocytes and NK cells
• Siglecs (sialic acid/immunolgobulin/lectin) are a family of I-type lectins binding to sialic acids on the cell surface
• They are a family of carbohydrate-binding proteins within the immunoglobulin superfamily
• Siglecs are all integral membrane proteins with extracellular domains consisting of unusual N-termial V-set Ig domains, followed by variable numbers of C2-set Ig domains
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.