CD124 Mouse anti-Human, Brilliant Violet 421, Clone: hIL4R-M57, BD Optibuild™

Description

• The hIL4R-M57 antibody specifically binds to the α subunit (IL-4R α) of the human Interleukin-4 Receptor complex (CD124)
• The human IL-4R α, or B cell stimulatory factor 1 receptor (BSF-1 receptor), is a 140kDa transmembrane glycoprotein that is expressed by B and T lymphocytes and a variety of other hematopoietic and non-hematopoietic cells and cell lines
• The cell surface IL-4R α chain binds IL-4 with high affinity and associates with either the common γ chain (IL-4R α/γ c; aka, type I IL-4R complex) or the IL-13 receptor alpha-1 subunit (IL-4R α /IL-13R α 1; aka, type II IL-4R complex) to form two distinct types of signal-transducing IL-4R complexes
• The type I IL-4 receptor complex specifically binds IL-4 whereas the type II IL-4R complex binds and transduces signals from either IL-4 or IL-13
• A truncated form of the IL-4Rα exists in soluble form in biological fluids
• In contrast to mice, in humans no distinct mRNA coding for sIL-4R has been described, suggested that human sIL4-R is exclusively produced by proteolytic cleavage of the cell surface receptor
• The serum levels of soluble IL-4R α appear to elevate in pathological situations such as allergy and parasitic infections
• Depending on the ratios of IL-4 and sIL-4Rα present in the local milieu, the sIL-4R α may augment or antagonize the activities of IL-4
• The immunogen used to generate the hIL4R-M57 hybridoma was soluble human IL-4R
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.