BDB743566
CD172a/b Mouse anti-Human, Brilliant Violet 711, Clone: SE5A5, BD Optibuild™
Manufacturer: BD Biosciences
Select a Size
| Pack Size | SKU | Availability | Price |
|---|---|---|---|
| Each of 1 | BDB743566-Each-of-1 | In Stock | ₹ 47,400.24 |
BDB743566 - Each of 1
In Stock
Quantity
1
Base Price: ₹ 47,400.24
GST (18%): ₹ 8,532.043
Total Price: ₹ 55,932.283
Antigen
CD172a/b
Clone
SE5A5
Conjugate
Brilliant Violet 711
Gene Alias
SIRP alpha/beta1, SIRPα/SIRPβ1, Signal Regulatory Protein α/β1
Immunogen
Human SIRP alpha extracellular domain Recombinant Protein
Quantity
50 μg
Primary or Secondary
Primary
Content And Storage
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification
Monoclonal
Concentration
0.2mg/mL
Formulation
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species
Mouse
Purification Method
Affinity Purified
Regulatory Status
RUO
Target Species
Human
Isotype
IgG1 κ
Description
- The SE5A5 monoclonal antibody specifically binds to a common epitope on CD172a/ SIRPα (90 kDa) and CD172b/ SIRPβ 1 (50 kDa)
- These transmembrane glycoproteins are members of the Signal Regulatory Protein (SIRP) family that, in turn, belongs to the Immunoglobulin superfamily
- The SIRP family is comprised of two subgroups, SIRPα and SIRPβ that are distinguished by the presence (α) or absence (β) of a cytoplasmic domain containing two immunoreceptor tyrosine-based inhibition motifs (ITIM)
- CD172a/ SIRPα is expressed on CD34+ stem/progenitor cells, cardiomyocytes, monocytes, macrophages, granulocytes, dendritic cells, and in the central nervous system
- It binds to CD47 and is implicated in mediating inhibitory signals via the ITIM/SHP-2 association
- CD172b/ SIRPβ 1 does not possess a cytoplasmic domain but instead the transmembrane domain contains a positively-charged residue that can interact with another transmembrane protein, DAP-12/KARAP
- DAP-12 has two immunoreceptor tyrosine-based activat ion motifs (ITAM) within its cytoplasmic domain that are thought to link CD172b to cellular activation signaling
- CD172b is expressed on myeloid cells, including peripheral blood monocytes and granulocytes
- It is not expressed on CD34+ cells
- CD172a and CD172b have complementary role s in signal regulation and may work together in tuning certain cellular responses to stimuli
- The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
- This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
- BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
- Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
- However, the spillover can be corrected through compensation as with any other dye combination
- BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
- This dye offers a very bright choice for the violet laser
- Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
- BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.
Compare Similar Items
Show Difference
Antigen: CD172a/b
Clone: SE5A5
Conjugate: Brilliant Violet 711
Gene Alias: SIRP alpha/beta1, SIRPα/SIRPβ1, Signal Regulatory Protein α/β1
Immunogen: Human SIRP alpha extracellular domain Recombinant Protein
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Human
Isotype: IgG1 κ
Antigen:
CD172a/b
Clone:
SE5A5
Conjugate:
Brilliant Violet 711
Gene Alias:
SIRP alpha/beta1, SIRPα/SIRPβ1, Signal Regulatory Protein α/β1
Immunogen:
Human SIRP alpha extracellular domain Recombinant Protein
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG1 κ
Antigen: CD268 (BAFF Receptor)
Clone: 11C1
Conjugate: Brilliant Violet 421
Gene Alias: __
Immunogen: __
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Refrigerate (2 to 8°C)
Classification: Monoclonal
Concentration: 0.2 mg/mL
Formulation: __
Host Species: Mouse
Purification Method: __
Regulatory Status: RUO
Target Species: Human
Isotype: IgG1 κ
Antigen:
CD268 (BAFF Receptor)
Clone:
11C1
Conjugate:
Brilliant Violet 421
Gene Alias:
__
Immunogen:
__
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Refrigerate (2 to 8°C)
Classification:
Monoclonal
Concentration:
0.2 mg/mL
Formulation:
__
Host Species:
Mouse
Purification Method:
__
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG1 κ
Antigen: CD268 (BAFF Receptor)
Clone: 11C1
Conjugate: Brilliant Violet 510
Gene Alias: BAFF Receptor, BAFF-R, BLyS receptor 3, BR3, TNFRSF13C
Immunogen: Human BAFF-R Transfected Cell Line
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Human
Isotype: IgG1 κ
Antigen:
CD268 (BAFF Receptor)
Clone:
11C1
Conjugate:
Brilliant Violet 510
Gene Alias:
BAFF Receptor, BAFF-R, BLyS receptor 3, BR3, TNFRSF13C
Immunogen:
Human BAFF-R Transfected Cell Line
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG1 κ
Antigen: CD268 (BAFF Receptor)
Clone: 11C1
Conjugate: Brilliant Violet 650
Gene Alias: BAFF Receptor, BAFF-R, BLyS receptor 3, BR3, TNFRSF13C
Immunogen: Human BAFF-R Transfected Cell Line
Quantity: 50 μg
Primary or Secondary: Primary
Content And Storage: Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification: Monoclonal
Concentration: 0.2mg/mL
Formulation: Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species: Mouse
Purification Method: Affinity Purified
Regulatory Status: RUO
Target Species: Human
Isotype: IgG1 κ
Antigen:
CD268 (BAFF Receptor)
Clone:
11C1
Conjugate:
Brilliant Violet 650
Gene Alias:
BAFF Receptor, BAFF-R, BLyS receptor 3, BR3, TNFRSF13C
Immunogen:
Human BAFF-R Transfected Cell Line
Quantity:
50 μg
Primary or Secondary:
Primary
Content And Storage:
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
Classification:
Monoclonal
Concentration:
0.2mg/mL
Formulation:
Aqueous buffered solution containing ≤0.09% sodium azide.
Host Species:
Mouse
Purification Method:
Affinity Purified
Regulatory Status:
RUO
Target Species:
Human
Isotype:
IgG1 κ