Vδ2 TCR Mouse anti-Human, Brilliant Violet 421, Clone: B6, BD Optibuild™

Description

• The B6 monoclonal antibody specifically recognizes the human variableδ2 subunit (Vδ2) of theγ/δT cell receptor (TCR)
• Theγ/δTCR is composed of two disulfide linked glycoproteins
• The delta chain is approximately 40-60 kDa and is restricted to peripheral blood T cells and thymocytes
• The majority of normal peripheral bloodγ/δT cells express a Vγ9[+]/ Vδ2[+] phenotype
• The reason for this selection in the T-cell repertoire is not well understood
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.