EphB2 Mouse anti-Human, Brilliant Violet 421, Clone: 2H9, BD Optibuild™

Description

• The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2)
• EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors
• EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B)
• Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems
• The EphB2 receptor functions as a chemodirectant in cellular migration
• EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, influencing cell fate, morphogenesis and organogenesis
• In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions
• Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation
• Ephrin-B2 molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes
• This interaction promotes monocyte differentiation into proinflammatory macrophages
• In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression
• These include intestinal stem cells (ISCs) which express high levels of EphB2
• The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2
• The antibody was conjugated to BD Horizon™ BV421 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max at 421nm, BD Horizon BV421 can be excited by the violet laser and detected in the standard Pacific Blue™ filter set (eg, 450/50nm filter)
• BD Horizon BV421 conjugates are very bright, often exhibiting a 10 fold improvement in brightness compared to Pacific Blue conjugates
• BV421 is a polymer-based dye excited by the violet laser and is one of the brightest fluorochromes offered by BD Biosciences
• Conjugates are typically 10 times brighter than Pacific Blue™ conjugates and are often as bright as or brighter than PE conjugates
• Due to nearly identical excitation and emission properties but different spillover characteristics, BD Horizon BV421, Pacific Blue™, and BD Horizon™ V450 cannot be used simultaneously.