EphB2 Mouse anti-Human, Brilliant Violet 605, Clone: 2H9, BD Optibuild™

Description

• The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2)
• EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors
• EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B)
• Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems
• The EphB2 receptor functions as a chemodirectant in cellular migration
• EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, influencing cell fate, morphogenesis and organogenesis
• In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions
• Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation
• Ephrin-B2 molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes
• This interaction promotes monocyte differentiation into proinflammatory macrophages
• In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression
• These include intestinal stem cells (ISCs) which express high levels of EphB2
• The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.