HVEM (CD270) Mouse anti-Human, Brilliant Violet 605, Clone: CW10, BD Optibuild™

Description

• The CW10 monoclonal antibody specifically binds to HVEM (Herpes virus entry mediator)
• HVEM is also known as CD270, Tumor necrosis factor receptor-like 2 (TR2), or LIGHT Receptor (LIGHT-R)
• CD270 is a type I transmembrane protein and member of the TNF Receptor superfamily
• It is encoded by TNFRSF14 (tumor necrosis factor receptor superfamily member 14)
• CD270 is expressed on T cells, B cells, NK cells, monocytes, granulocytes, and some dendritic cells
• CD270 binds to CD258/LIGHT, CD272/BTLA, and glycoprotein D of Herpes simplex viruses HSV-1 and HSV-2
• CD270-LIGHT interactions can reportedly transduce costimulatory signals for T cells whereas CD270-BTLA interactions can deliver inhibitory signals to T cells
• This antibody is conjugated to BD Horizon™ BV605 which is part of the BD Horizon Brilliant™ Violet family of dyes
• With an Ex Max of 407nm and Em Max of 602nm, BD Horizon BV605 can be excited by a violet laser and detected with a standard 610/20nm filter set
• BD Horizon BV605 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an Em max at 605nm
• Due to the excitation of the acceptor dye by the green (532 nm) and yellow-green (561 nm) lasers, there will be significant spillover into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers
• BD Horizon BV605 conjugates are very bright, often exhibiting brightness equivalent to PE conjugates and can be used as a third color off of the violet laser
• BV605 is a tandem fluorochrome that combines BD Horizon BV421 and Cy™3.5
• These conjugates are very bright, exhibiting similar brightness to equivalent PE conjugates
• Due to the excitation of Cy3.5 by the green (532nm) and yellow-green (561nm) lasers, there will be significant spillover of the BD Horizon BV605 signal into the PE and BD Horizon PE-CF594 detectors off the green or yellow-green lasers.