EphB2 Mouse anti-Human, BUV395, Clone: 2H9, BD Optibuild™

Description

• The 2H9 monoclonal antibody specifically binds to the Ephrin Type-B Receptor 2 (EphB2)
• EphB2 is a type I transmembrane glycoprotein that belongs to the Eph receptor family of tyrosine kinase receptors
• EphB2 serves as a cell surface receptor tyrosine kinase for membrane-anchored ligands referred to as type B ephrins (ephrin-B)
• Transmembrane ephrin-B family members are key regulators of embryogenesis including development of the nervous and vascular systems
• The EphB2 receptor functions as a chemodirectant in cellular migration
• EphB2/ephrin-B interactions orchestrate cell positioning by regulating cellular adhesion and repulsion during development, influencing cell fate, morphogenesis and organogenesis
• In the adult body, Eph receptor signaling plays major roles in regulating the architecture and physiology of different tissues under normal as well as disease conditions
• Ephrin-B1 and ephrin-B2 levels are upregulated in the vasculature during inflammation
• Ephrin-B2 molecules that are localized to the luminal endothelial surface can signal through the EphB2 which is expressed by monocytes
• This interaction promotes monocyte differentiation into proinflammatory macrophages
• In the intestinal epithelium, EphB2/ephrin-B interactions regulate both cell positioning and tumor progression
• These include intestinal stem cells (ISCs) which express high levels of EphB2
• The 2H9 antibody reportedly blocks the interaction of EphB2 with ephrin ligands and crossreacts with mouse EphB2
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.