CD31 Mouse anti-Human, BUV395, Clone: M89D3, BD Optibuild™

Description

• The M89D3 monoclonal antibody specifically recognizesCD31, which is also known as platelet endothelial cell adhesion molecule - 1 (PECAM-1), a single chain, type I transmembrane protein of approximately 130-140 kDa
• CD31 is a member of the immunoglobulin supergene family, its extracellular region is composed of 574 amino acids and contains six C2-type Ig domains, its intracellular region is composed of 118 residues
• CD31 (PECAM-1) is expressed on endothelial cells, platelets, monocytes, neutrophils and NK cells
• It has also been observed on subsets of T cells, but not on circulating B cells
• It has been implicated in a number of cellular phenomena, including vascular wound healing, angiogenesis, and transendothelial migration of leukocytes and platelet aggregation in inflammatory responses
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.