CD31 Mouse anti-Human, Brilliant Violet 650, Clone: M89D3, BD Optibuild™

Description

• The M89D3 monoclonal antibody specifically recognizesCD31, which is also known as platelet endothelial cell adhesion molecule - 1 (PECAM-1), a single chain, type I transmembrane protein of approximately 130-140 kDa
• CD31 is a member of the immunoglobulin supergene family, its extracellular region is composed of 574 amino acids and contains six C2-type Ig domains, its intracellular region is composed of 118 residues
• CD31 (PECAM-1) is expressed on endothelial cells, platelets, monocytes, neutrophils and NK cells
• It has also been observed on subsets of T cells, but not on circulating B cells
• It has been implicated in a number of cellular phenomena, including vascular wound healing, angiogenesis, and transendothelial migration of leukocytes and platelet aggregation in inflammatory responses
• The antibody was conjugated to BD Horizon™ BV650 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 650nm
• BD Horizon BV650 can be excited by the violet laser and detected in a filter used to detect APC-like dyes (eg, 660/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV650 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 650 nm
• Due to the excitation and emission characteristics of the acceptor dye, there will be spillover into the APC and Alexa Fluor™ 700 detectors
• BV650 will have moderate spillover into the BD Horizon™ BV711 detector.