CD24 Rat anti-Mouse, BUV395, Clone: M1/69, BD Optibuild™

Description

• The M1/69 monoclonal antibody specifically binds to CD24 (Heat-Stable Antigen, HSA or HsAg), a variably glycosylated, glycosyl-phosphatidylinositol-anchored membrane protein expressed on erythrocytes, granulocytes, monocytes, lymphocytes, and neurons
• Hematopoietic stem cells of the embryonic yolk sac and fetal liver express CD24
• Levels of expression of CD24 vary during differentiation of the T and B cell lineages
• In the bone marrow, hematopoietic progenitors acquire CD24 expression upon commitment to the B-lymphocyte lineage
• Immature B cells in the bone marrow express low CD24 levels whereas peripheral B lymphocytes express intermediate to high levels of CD24
• The level of CD24 expression has been reported to rise upon activation of splenic B cells with LPS, but not with CD154 (CD40 Ligand)
• The majority of thymocytes express high levels of CD24, while most mature thymic and peripheral T lymphocytes do not express CD24
• In contrast, TCR-bearing thymocytes which emigrate to the spleen are CD24+
• Dendritic cells of the thymus, spleen, liver, and epidermal Langerhans cells have also been reported to express CD24
• CD24 is not expressed by NK cells
• CD24 is involved in the costimulation of CD4+ T cells by B cells, it is a co-inducer of in vitro thymocyte maturation, and it is a ligand of CD62P (P-selectin)
• While the monoclonal antibodies 30-F1, M1/69, and J11d all react with CD24, they show subtle differences in the level of staining of different lymphocyte populations
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.