CD18 Rat anti-Mouse, BUV395, Clone: M18/2, BD Optibuild™

Description

• The M18/2 antibody specifically recognizes the commonβ2 chain of LFA-1 (CD11a/CD18,αLβ2 integrin), Mac-1 (CD11b/CD18,αMβ2 integrin), and gp150, 95 (CD11c/CD18,αXβ2 integrin)
• Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrinαchains (CD11a, CD11b, and CD11c)
• Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells
• Theβ2 integrins are important mediators of leukocyte-endothelium interactions
• It has been reported that M18/2 antibody blocks in vivo /metastasis of the LB lymphoma to the spleen and that it blocks in vitro formation of aggregates of LB cells and splenocytes
• However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts
• Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3
• The antibody was conjugated to BD Horizon™ BUV395 which is part of the BD Horizon Brilliant™ Ultraviolet family of dyes
• This dye has been exclusively developed by BD Biosciences to have minimal spillover into other detectors, making it an optimal choice for multicolor flow cytometry
• With an Ex Max at 348 nm and an Em Max at 395 nm, BD Horizon BUV395 can be excited with a 355 nm laser and detected with a 379/28 filter
• BUV395 is a UV-excitable dye that has been developed exclusively by BD Biosciences
• With an excitation max of 348 nm and emission max of 395 nm, BUV395 can be excited by the 355nm laser and detected in a 379/28 filter
• This dye is optimal for multicolor flow cytometry because it has little to no spillover into other detectors.