CD18 Rat anti-Mouse, Brilliant Violet 711, Clone: M18/2, BD Optibuild™

Description

• The M18/2 antibody specifically recognizes the common β 2 chain of LFA-1 (CD11a/CD18, α Lβ 2 integrin), Mac-1 (CD11b/CD18, α Mβ 2 integrin), and gp150, 95 (CD11c/CD18, α Xβ 2 integrin)
• Expression of CD18 is limited to leukocytes, where it is widely distributed in consort with the three integrin α chains (CD11a, CD11b, and CD11c)
• Among splenocytes, NK cells have the highest density of CD18, and T lymphocytes express a higher density than the remaining cells
• The β 2 integrins are important mediators of leukocyte-endothelium interactions
• It has been reported that M18/2 antibody blocks in vivo metastasis of the LB lymphoma to the spleen and that it blocksi in vitro formation of aggregates of LB cells and splenocytes
• However, other reports indicate that mAb M18/2 has no effect on CTLmediated killing, adherence of C3bi-sensitized erythrocytes to Mac-1, antigen-specific binding of T cells to antigen-producing cells, or rejection of cardiac allografts
• Recent in vitro studies indicate that M18/2 antibody stimulates adhesion of Mac-1 to its ligands C3bi and ICAM-1, and it stimulates adhesion of LFA-1 to ICAM-1, but it has no effect upon the interactions of LFA-1 with ICAM-2 nor ICAM-3
• The antibody was conjugated to BD Horizon™ BV711 which is part of the BD Horizon Brilliant™ Violet family of dyes
• This dye is a tandem fluorochrome of BD Horizon BV421 with an Ex Max of 405nm and an acceptor dye with an Em Max at 711nm
• BD Horizon BV711 can be excited by the violet laser and detected in a filter used to detect Cy™5.5 / Alexa Fluor™ 700-like dyes (eg, 712/20nm filter)
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• However, the spillover can be corrected through compensation as with any other dye combination
• BV711 is a tandem fluorochrome of BD Horizon BV421 and an acceptor dye with an emission maximum at 711 nm
• This dye offers a very bright choice for the violet laser
• Due to the excitation and emission characteristics of the acceptor dye, there may be moderate spillover into the Alexa Fluor™ 700 and PerCP-Cy5.5 detectors
• BV711 will also have moderate spillover into the BD Horizon Brilliant™ Violet 786 detector.